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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in many tumors, which leads to chromosome instability, and thus may perhaps add to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.

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This guidebook outlines even more achievable optimization of cellular and immunoassay parameters to be sure the best possible success are...

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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...

Antibodies bound to the blocking peptide now not bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody compared to the antibody on your own, you can see which staining is distinct.

Goal Details Cyclin E belongs for the remarkably conserved cyclin loved ones, whose associates are characterised by a spectacular periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation styles which add for the temporal coordination of each and every mitotic function. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary which is degraded as cells progress by means of S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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